| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jones, Katherine E (13)
|
NITWST |
|
5:22.31 |
- |
| 2 |
Choroser, Beatrice C (14)
|
NITWST |
5:23.06 |
5:22.60 |
-0.46 |
| 3 |
Bautz, Michelina P (13)
|
NITWST |
|
5:23.70 |
- |
| 4 |
Chugh, Katie F (13)
|
NIEAST |
|
5:41.64 |
- |
| 5 |
Castonguay, Laura J (13)
|
NITWST |
|
5:50.36 |
- |
| 6 |
Marshall, Olivia J (13)
|
NIEMAC |
5:55.95 |
5:57.44 |
1.49 |
| 7 |
Johnson, Emma E (13)
|
NITWST |
|
6:11.88 |
- |
| 8 |
Musterait, Madeline A (13)
|
NITWST |
|
6:16.14 |
- |
| 9 |
Kinderman, Gabrielle C (13)
|
NITWST |
|
6:29.32 |
- |
| 10 |
Penn, Emily G (13)
|
NITWST |
|
6:30.24 |
- |
| 11 |
Lane, Morgan E (13)
|
NITWST |
|
6:35.67 |
- |
| 12 |
Hogle, Brooke E (13)
|
NITWST |
|
7:17.94 |
- |